Page 6 - 07利用畜牧沼液配製高品質有機液肥及作物之肥培應用

3. Analysis and application of microbiology in liquid fertilizer

(1)Microbiology analysis in the different liquid fertilzers

After sampling 1 ml of liquid fertilizers, and 9 ml distilled water was added. The

mixture was shaked. And then the 1 ml mixture was sampled, and 9 ml distilled water

was added. After multiple dilution, the liquid was smeared on the surface of solid

medium. Three replicates were proceeded. After that, the solid medium was placed to

o
the growth chamber that containing constant temperature (27 C). After the

microbiology was observed apparently, the total microbiological amount was

calculated averagely for three replications. The calculative formula was as below,

Average amount of microbiology= Average microbiology= average microbiology of

three replications 1/dilutive fold 10 ( unit: CFU/ml).

(2)The test of microbiology isolated from liquid fertilizer for the antibiosis of

mango anthraconosis

Anthrax is a kind of important disease on mango (Liu et al., 2014). Some method

were found to to inhibit anthrax effectively (Jiao, et al., 2018). At first, the processing

groups were set as follow. The isolated microbiology strain from liquid fertilizer was

inoculated to the medium containing agar that located the 1/4 right district. The

medium were cultured in the growth chamber containing temperature 30 C for 1 day.
o

And then the anthraconosis srain was inoculated on the 1/4 left district. And then the

medium was continuously placed in the growth chamber that the temperature was set

at 30 C. On the other hand, the treatment of check (CK) was set that only inoculating
o
anthraconosis srain on the 1/4 left district of medium and there were no isolated

5